Gemstones and Refraction Liquids

sapphire - refraction liquids
With a high refractive index of 1.741, diiodomethane (methylene iodide) is an excellent refraction liquid for testing sapphires for diffusion treatments as well as viewing their inclusions more clearly. “Blue Sapphire” by Jan-Peter Geringer. Licensed under CC By-SA 4.0.

Gemologists use liquids with high refractive indices (RI) to ascertain gemstone RIs and, thus, help identify and distinguish them. In addition, any inclusions in a gem may appear more clearly when it’s immersed in one of these liquids. Refraction liquids can also help identify diffusion treatments.

Advantages and Disadvantages of Commonly Used Refraction Liquids

Although quite useful, these refraction liquids have some drawbacks. Gemologists, especially novices, should consider their use carefully. These liquids can be expensive, dangerous, poisonous, and/or flammable. However, less aggressive, less expensive dense oil alternatives do exist. For example, glycerol or glycerin oil has an RI of 1.47.

While other dense oils have desirable RIs, many also have very strong scents. Cleaning a gem dipped in these oils can prove difficult. Another problem you might encounter with these alternatives is a lack of purity. When tested with a refractometer, some allegedly pure oils had RIs so low they could only be dilutions. (Editor’s note: Some natural oils also simply have variable properties, including RIs). As a rule, anytime you use a new refraction liquid in your lab, check a drop of it in your refractometer to confirm its RI before you immerse your gems in it.

Let’s examine the following table of properties of liquids commonly used to test gemstone RIs.

Table 1: Properties of Commonly Used Refraction Liquids

Carbon Tetrachloride1.460CCl4 D3 (1)US$96/liter
Toluene1.500C7H8 or C6H5CH3 D2US$34/liter
Monochlorbenzene1.526C6H5Cl D5D4US$55/liter
Monobromobenzene, 1-Bromobenzene1.560C6H5Br D5
O-Toluidine, O-Methylaniline, 2-Methylaniline1.570C7H9N D2
Bromoform1.590Br3CH D3 (1)D4US$315/kg
1-Bromonaphthalene1.660C10H7Br D5US$34/100 ml
Monoiodonaphtalene1.705C10H7I D5
Diiodomethane (Methylene Iodide)1.741CH2I2 D3 (1)

Table 2: Hazard Symbol Key

Dangerous to the Environment
highly flammable - refraction liquidsHighly Flammable
toxic - refraction liquidsToxic
harmful - refraction liquidsHarmful

To learn more about these and other chemical hazards as well as safety precautions, consult this article.


As you can see, we have at least nine products with RIs ranging from 1.460 to 1.741. For a gemological lab, you should have 200 ml of each liquid on hand. Why do you need multiple liquids? You may need refraction liquids with different RIs to see inclusions and determine the RIs of various gemstones.

If you don’t want to purchase this many costly and dangerous liquids, you have another option. Look at the details for 1-bromonaphthalene in Table 1. Note that it has a good balance between price and RI. (The color ranges from slightly yellow to deep brownish-yellow). In addition, this product is slightly soluble in water but miscible with alcohol, ether, benzene, and chloroform. Using the purest alcohol you can find, you can dilute 1-bromonaphthalene. Thus, you can create a set of refraction liquids that range from 1.400 to 1.660. To do this, dilute slowly. Take a sample drop of the liquid after each dilution to test in your refractometer. Each time you create a solution with a RI you want to keep, save a batch and label. Then, move on to your next dilution.

Unfortunately, high RI liquids such as monoiodonaphtalene and diiodomethane don’t have easy substitutions.

Safety Rules for Refraction Liquids

Safety first! Never forget these important rules:

  • Avoid skin contact with the liquids.
  • Use goggles and a face mask.
  • Keep fresh air in circulation, even by using a small fan if necessary.
  • Keep children and animals away from your workspace when using these liquids. (In fact, if you work in a home lab and have children, you probably shouldn’t use these liquids at all).
  • For each refraction liquid, use one pipette with a paper tag indicating the respective RI liquid.
  • Don’t mix the tools between liquids or you’ll ruin your calibration work.

Partial Immersion Technique

To inspect inclusions in a gemstone, you don’t need to immerse it in the refraction liquid completely. Instead, you can use the partial immersion technique.

To do this, place only a drop of liquid over the gem, then observe it.

With this technique, you’ll minimize your risk of skin contact and vapor exposure, as well as reduce your use of costly refraction liquids. Figure 1 illustrates the partial immersion technique.

partial immersion technique - refraction liquids
Figure 1: The partial immersion technique.

Total Immersion Technique

With total immersion, you can use a mount with a 45° mirror, as shown in Figure 2.

total immersion technique - refraction liquids
Figure 2: A setup for total immersion incorporating a mount with a 45° mirror.

I prefer using an adapted plastic black box, as shown in Figure 3.

black box for total immersion testing - refraction liquids
Figure 3: My adapted black box that I use for examining gemstones with the total immersion technique.

Finally, to jog your memory, Figure 4 shows you what gemstones will look like when totally immersed in refraction liquids.

total immersion results - refraction liquids
Figure 4: What you can expect to see when the gemstone RI (RIG) is greater, near, equal, or less than the liquid RI (RIL) under total immersion testing.

About the author
Dr. Raul Berenguel, PhD.
Dr. Raul Berenguel holds degrees in History (Scientific Branch, 2008) and Contemporary Art (PhD, 2012) from the Universidade Aberta. He has specific expertise in the fields of history, gemology, and contemporary art. In addition, he’s also an expert in applying traditional gemological techniques to investigating art objects made from gem materials.
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